Graphpad prism 6 two sets of symbols one graph

#GRAPHPAD PRISM 6 TWO SETS OF SYMBOLS ONE GRAPH PLUS#

Phenotypical and functional characterization of CD4 +CD25 + T cell subset from human peripheral blood. Supporting Information Files in this Data Supplement: SI Figure 6 SI Figure 7 SI Figure 8 SI Figure 9 SI Figure 10 SI Figure 11 SI Figure 12 SI Figure 13 SI Text SI Figure 6ДЇig. Statistical significance of differences between groups was evaluated by an unpaired Student's t test using the GraphPad Prism software. The histograms represent the mean and SD values of three independent experiments using cells from three different donors.

#GRAPHPAD PRISM 6 TWO SETS OF SYMBOLS ONE GRAPH PLUS#

Forty-four conjugates T H alone plus anti-TGF-ОІ Ab, 43 conjugates T H plus Treg plus the isotype control Ab, and 41 conjugates T H plus Treg and plus anti-TGF-ОІ Ab were scored. ( D) Quantification of T H IFN-Оі polarization toward APC in the culture conditions described in C. White arrows indicate IFN-Оі accumulation white circles mark T cell contour. In b and e, cells were treated with an isotype control Ab. In a, d, c, and f, cells were treated with anti-TGF-ОІ mAb at time 0 of conjugate formation. After 2-h 30-min incubation at 37вЊ, cells were stained for IFN-Оі. ( C) EBV-B cells (red) were conjugated with either T H alone (unstained, a, d) or with T H plus Treg (green, b, c, e, f) at 1:1:1 ratio. A total of 48 three-cell conjugates (plus Treg) and 48 conjugates (without Treg) were scored. ( B) Quantification of T H GM130 polarization toward APC in the culture conditions described in A.

White arrows indicate GM130 localization white circles mark T cell contour. T H (unstained) were added for an additional 15 min at 1:1:1 ratio. ( A) EBV-B cells (red) were either kept in culture alone ( a, c) or conjugated with Treg (green, b, d) for 2 h 30 min. Inhibition of polarized IFN-Оі secretion in T H cells is mediated by TGF-ОІ production. In D, 82 three-cell conjugates plus Treg and 60 conjugates without Treg were scored for GM130 88 three-cell conjugates plus Treg and 91 conjugates without Treg were scored for tubulin. In C, 66 three-cell conjugates plus Treg and 85 conjugates without Treg were scored for GM130 60 three-cell conjugates plus Treg and 63 conjugates without Treg were scored for tubulin. ( C and D) Quantification of T H GM130 and tubulin polarization toward APC after 15 min incubation ( C) or after 2-h 30-min incubation ( D). White arrows indicate localization of MTOC or GM130 white circles mark T cell contour. After 15-min incubation at 37вЊ ( A) or 2-h 30-min incubation at 37вЊ ( B), cells were stained for either GM130 ( Aa and Ba) or tubulin ( Ab and Bb). ( A and B) EBV-B cells (red) were conjugated with T H (unstained) in the presence of Treg (green) at 1:1:1 ratio. EBV-B cells were conjugated with T H for different times either in the presence or in the absence of Treg. Inhibition of secretory machinery polarization occurs in a time-dependent fashion. Statistical significance of differences between groups was evaluated by an unpaired Student's t test with GraphPad Prism software. Data are from three independent experiments performed by using cells from two different donors. ( Lower) Percentage of T H in conjugate that exhibit i increase. ( Upper) Percentage of Indo-1-loaded T H cells in conjugate.

Indo-1-loaded T H were conjugated at the ratio 1:1:1 (T H Indo-1 + : APC : Treg or control T H). ( B) APC pulsed with either 100 ng/ml or 1 ng/ml superantigens were precultured with Treg or control T H for 2 h 30 min. ( Lower Right) Mean fluorescence intensity of 405/530 emission ratio in T H cells at different time points after conjugate formation. ( Lower Left) Percentage of T H in conjugate that exhibit i increase. ( Upper) Percentage of Indo-1-loaded T H cells in conjugate at the indicated time points. ( A) APC were either unpulsed or pulsed with 100 ng/ml, 10 ng/ml, or 1 ng/ml superantigens. The following cellular ratio was used 1:1:1 (T H Indo-1 + : APC : Treg or control T H). Indo-1-loaded T H cells were conjugated with EBV-B cells in the presence of either unstained T H or unstained Treg. Sustained i increase in T H cells is unaffected in the presence of Treg.

Graphpad prism 6 two sets of symbols one graph